Biomedical Engineering Reference
In-Depth Information
CHAPTER 9
Physiological Cellular Reactions
Detection on Biosensor Surfaces:
A Fractal Analysis
Chapter Outline
9.1 Introduction
223
9.2 Theory
224
9.2.1 Single-Fractal Analysis
224
Binding Rate Coefficient
224
Dissociation Rate Coefficient
225
9.2.2 Dual-Fractal Analysis
225
Binding Rate Coefficient
225
9.3 Results
226
9.4 Conclusions
252
9.1 Introduction
The real-time monitoring of cells should help reveal the mysteries of modern cell biology
(
Ziblat et al., 2006
). These authors point out that though the surface plasmon resonance
(SPR) is a useful technique, it is not well suited for analyzing the reactions
in vivo
or
in situ
.
They, therefore emphasize the need to develop new experimental techniques that would mon-
itor quantitatively in real time the dynamics between molecules and their cognate receptors in
cells. They have developed a novel SPR method based on FTIR.
Fang et al. (2006)
also reinforce this view by pointing out that the ability to analyze living
cells in their natural and physiological state is essential to understand the biological functions
of cellular targets. This should also assist in drug discovery and in its development. These
authors have summarized the principles that are involved in current cell-base arrays that
include measuring of a specific cellular event that includes second-messenger generation to
the translocation of a particular target tagged with a fluorescent label. One may also include
the expression of a reporter gene, and the alteration of a particular phenotype (
Blake, 2001;
Taylor et al., 2001; Abraham et al., 2004
).
Fang et al. (2006)
report that optical biosensors