Biomedical Engineering Reference
In-Depth Information
CHAPTER 6
Binding of the Same Analyte to
Different Biosensor Surfaces
Chapter Outline
6.1 Introduction
129
6.2 Theory
131
6.2.1 Single-Fractal Analysis
131
Binding Rate Coefficient
131
Dissociation Rate Coefficient
131
6.2.2 Dual-Fractal Analysis
132
Binding Rate Coefficient
132
6.1 Introduction
A fractal analysis is presented on the binding and dissociation (if applicable) kinetics of the
same analyte to different biosensor systems. The intent is to note if (a) further physical
insight into these types of systems could be gained, and (b) if there were some reasons
why particular ranges of fractal dimensions (degree of heterogeneity) existed on the bio-
sensor surface and in the binding rate coefficients. These physical insights could be of value
for analyte-receptor systems, in general, but particularly so in those analyte-receptor sys-
tems that exhibit biomedical/medical applications such as glucose, thrombin,
a
-fetoprotein
(AFP) and carcinogenic analytes or those analytes that may be used as cancer biomarkers.
The examples selected for analysis and comparison were done so at random from those that
were available in the literature. Simple criteria were used in the selection procedure. There
should, of course, be at least two examples available for the same analyte for comparison
purposes. More than two examples of the same analyte would be even better for comparison
purposes. Concentration (or any other measurable entity made quantitative by the biosensor
for that particular analyte-receptor biosensor system) versus time should be available, and
