Design and Development of In Vivo Sensor Systems: The Long and Tortured Road to a Self-Contained, Implantable Glucose Sensor for Diabetes Management - Designing Receptors for the Next Generation of Biosensors - page 219

Boronic Acid 1
Boronic Acid 2

1.5

1.0

0.5

0.0

[Glucose] mg/dL

Fig. 4 ARS competition binding assay. Glucose competition curves showing normalized fluores-
cence intensity versus glucose concentration for boronic acid 1 and boronic acid 2 in a physiologi-
cal buffer at neutral pH

Fig. 5 Structures of boronic acid 1 (a) and boronic acid 2 (b) evaluated for binding performance
in a glucose competition binding assay

Following boronic acid-glucose binding affinity prescreening, we synthesized a
library of DBAs using the selected, candidate boronic acids. Each DBA in the
library was subsequently screened against each candidate saccharide mimic.

4 Glucose-Competitive DBA Binding Environment (iDIOL)

Diols, in the form of immobilized saccharide mimics (iDIOLs), have been used by
our group as a glucose-competitive, DBA binding environment in a competitive
binding assay that serves as the prototype for the ultimate mass-sensitive, in vivo
glucose sensor. Thus, the second critical step required for the demonstration of the
self-contained glucose sensing system was the selection of the glucose-competitive