Biomedical Engineering Reference
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as foods, clinical fluids, and environmental specimens. AMPs and AMP-based
materials can then be downselected to those with minimal matrix effects.
We anticipate that information technology will play a leading role in the
development of future AMP-based assays. With increasing numbers of AMPs
being used in multiplexed assays, the ability to store libraries of binding patterns
and the ability to retrieve information necessary to deconvolute data from unknown
samples will be imperative. Furthermore, we foresee the day when biosensor assays
can be custom tailored to virtually any desired specificity (e.g., order-, family-,
genus-, or species-level discrimination) by selecting different sets of AMPs (and
linker chemistry) with appropriate recognition characteristics. Such AMP-based
biosensors have the potential to expand detection capabilities significantly beyond
what is currently possible by rapid screening of unknown samples to detect not only
well-characterized microbial
targets, but
those that are unknown or newly
emergent.
Acknowledgments This work has been funded by Joint Science & Technology Office for
Chemical & Biological Defense/Defense Threat Reduction Agency and the Office of Naval
Research (ONR). SND was supported by the Naval Research Enterprise Intern Program through
ASEE and ONR. The views expressed here are those of the authors and do not represent the
opinions of the U.S. Navy, the U.S. Department of Defense, or the U.S. Government.
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