Biomedical Engineering Reference
In-Depth Information
17.10 Prolonged localization of implanted BMNCs in ischemic tissues
by NS. (A) Co-localization of BMNCs with NS and LA in-vivo. Murine
BMNCs derived from EGFP-transgenic mice were transplanted together
with LA or NS into the thighs in the hind limb ischemic model. Cores of
NS and LA containing rhodamine B (orange) were used to indicate
localization of the injected microspheres in ischemic tissues. Tissue
sections 7 days after transplantation of LA+BMNCs (a) or NS+BMNCs (b)
were counterstained with DAPI (blue), and merged images of DAPI, GFP
and rhodamine B are shown. BMNCs (green) were observed as densely
clustered around NS (b) but not LA (a). Scale bars: 100mm. (B)
Quantitative evaluation of implanted cells existing in ischemic tissues.
Quantitative analysis of intramuscular GFP was performed 3, 7 and
14 days after transplantation. BMNCs were derived from EGFP-
transgenic mice. BMNCs were transplanted alone or together with LA or
NS into ischemic thigh muscles. Intramuscular GFP values of whole
thigh muscles were corrected for total protein and expressed in arbitrary
units (n = 6 in each group). *P
<
0.05 for the NS+BMNCs group
compared to the BMNCs alone and LA+BMNCs groups. GFP
concentration in normal murine muscle was measured as background
(BG) (Mima et al., 2012). Copyright 2012, Plos ONE.
ischemic thigh tissue 7 days after transplantation. Intramuscular levels of
GFP derived from transplanted BMNCs were consistently and significantly
higher in the group injected with NS than that injected with LA or BMNCs
alone at 3, 7 and 14 days after implantation, while GFP levels were not
