Biomedical Engineering Reference
In-Depth Information
FIGURE 6.2
Scanning electron microscopy (SEM) images of spherical calcium phosphate formed on immobilized FC type
1 on titanium specimen with AFP layer. The surface states FC/AFP (A) and FC/Anodic oxidation (AO) (B) after
exposure to SBF for 1 h (C) and 24 h (D)
[47]
.
“Silanization” is a simple technique employed to immobilize RGD peptide sequence covalently
onto titanium surface. The technique involves coating the surface with silane molecules through
chemical treatment and self-assembly. This silane molecule acts as a “bridging molecule” between
the substrate and the biomolecules. In a study using 3-aminopropyltriethoxysilane (APTES) chemis-
try, RGD sequence was bound to Ti-6Al-4V surface
[49]
.
In-vivo
study in canines showed improved
mechanical fixation with RGD-coated implants
[50]
. In a similar study, arginine-glycine-aspartic
acid-cysteine (RGDC) peptide sequence was immobilized on titanium surface through covalent addi-
tion by silanization technique using APTES chemistry
[51]
. Primary calvarial osteoblasts cultured
on RGDC-coated titanium surface showed increased cell attachment, morphology, proliferation, and
osteocalcin messenger RNA (mRNA) expression than the bare titanium surface
[51]
. These studies
demonstrate that silanization technique is not only simple and cost-effective but also can be success-
fully used for covalent binding of cell-adhesive peptide motifs or other osteoinductive growth factors
on titanium surface for implant applications.
