Biomedical Engineering Reference
In-Depth Information
FIGURE 12.7
SEM of hUCMSC attachment on: (A) CPC control and (B) CPC-chitosan-fiber scaffold. Cells are designated as
“C,” which anchored to CPC in (A), and to the fibers in the scaffold in (B). Cells developed long, cytoplasmic
extensions “E,” shown in (C) at a higher magnification, attaching firmly to the fiber in the CPC-chitosan-fiber
scaffold.
Adapted from Ref.
[45]
with permission.
were harvested as described in the literature
[48-52]
. Cells were cultured in a low glucose Dulbecco's
modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/
streptomycin (PS) (Invitrogen, Carlsbad, CA). Two materials were seeded with hUCMSCs: CPC con-
trol and CPC-chitosan-fiber; 150,000 cells
[53]
were diluted into 2 ml of media and added to each
well containing a CPC disk.
Figure 12.7
shows SEM micrographs of hUCMSCs seeded on: (A) CPC
control and (B) CPC-chitosan-fiber (with 20% by volume of fibers). In (A), hUCMSCs (designated
as “C”) had polygonal shapes and were anchored on CPC. In (B), cells attached to the fibers of CPC-
chitosan-fiber scaffold. The cells had developed cytoplasmic extensions “E,” which are visible in (A)
and (B), and are shown in (C) attaching to the fiber “F.”
