Biomedical Engineering Reference
In-Depth Information
FIGURE 11.1
The micropatterns designed using the CLEWIN software program. The patterns were designed from 10 to
40
μ
m in width and were repeated in quartets throughout the entire mask design.
Germany). Ten percent of Sylgard 184 curing agent was mixed with 90% of Sylgard 184 base sili-
cone elastomer and was mixed thoroughly. The air bubbles entrapped in the mixture were removed
under vacuum. The PDMS solution was poured over the “master” and left to cure overnight (
Figure
11.3
). The cured PDMS stamp was removed from the “master” and used in the subsequent steps.
