Biomedical Engineering Reference
In-Depth Information
Days post-anthesis
β keto reductase activity
Storage lipids
FIGURE 10.4 Relationship between deposition of lipids and β-keto reductase activity in a
developing oil seed. (Adapted from Slabas et al. 54 )
TABLE 10.5
cDNA/Genes Cloned to Date Related to Lipid Synthesis
Lipid Synthesis Gene
Source of cDNA and/or Gene Cloned
Acyl carrier protein (ACP)
Spinach leaf ACP I, barley leaf ACP I, barley leaf
ACP II, barley leaf ACP III, Arabidopsis leaf,
Brassica campestris seed, B. napus seed
Acetyl-CoA carboxylase
Carrot, wheat, maize, rapeseed, Arabidopsis
3-Ketoacyl-ACP synthase (KAS)
Barley leaf KAS 1, castor seed KAS 1
Enoyl-ACP reductase (NADPH)
B. napus seed, tobacco, petunia, Arabidopsis leaf
3-Ketoacyl-ACP reductase (NADPH)
Arabidopsis leaf, Cupheas lanceolata
∆9-Desaturase (soluble)
Castor seed, cucumber, safflower seed, B. rapa,
jojoba, B. napus seed
∆6-Desaturase (membrane-bound)
∆15-Desaturase (membrane-bound)
Acyl-ACP thioesterase
Safflower, rapeseed
Acyl-ACP thioesterase (medium-chain specific)
California bay laurel
Glycerol-3-P acyltransferase
Pea leaf, squash leaf, Arabidopsis leaf, cucumber
Adapted from Slabas et al. 54
soap and detergent industry and for various edible products as confectionery fats,
or as feedstock for oleochemicals. Table 10.6 lists the status of other transgenic
oilseeds nearing release to farmers. At the University of Saskatchewan, Canada,
genetic engineering techniques are being applied to modify flax fatty acids. DuPont
researchers have produced soybean lines with seed oil compositions of between
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