Biomedical Engineering Reference
In-Depth Information
9 The Effect of Oxidative
Enzymes in Foods
David S. Robinson
TABLE OF CONTENTS
Introduction
Occurrence in Foods
Oxygen Reactivity
Effects on Shelf Life
Lipoxygenases
Occurrence
Oxidation of Polyunsaturated Fatty Acids
Mode of Action
Co-Oxidation
Manipulation of Factors to Enhance Shelf Life
Peroxidases
Free Radical Generators
Peroxidase Activity
Effects on Shelf Life
Thermostability
Oxidatic Activity
Polyphenol Oxidases
Enzymic Browning
Monophenolase and Diphenolase Activity
Assays and Inhibitors
References
INTRODUCTION
O CCURRENCEIN F OODS
Oxidative enzymes through the action of oxygen are of increasing interest to the
food scientist due to their effect on both the color and flavor of plant foods. Polyphe-
nol oxidases are responsible for enzymic browning often coupled to the loss of
vitamin C in a wide range of vegetables, fruits, and juices. Ascorbic acid oxidase
also catalyzes the oxidation of ascorbic acid to dehydroascorbic acid which can then
degrade further to non-enzymic browning products. The effects of peroxidases,
although visually less obvious, may be equally damaging to food quality as this group
 
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