Biomedical Engineering Reference
In-Depth Information
space in between the clustered crystals of nCS (
Figure 19.9
) and creating macropores within the
scaffold.
The integrity of the 50%, 20% and 15% alginate, nCS scaffolds was lost completely after 24 h.
Although the 10% and 5% alginate, nCS scaffolds still kept their shape, their mechanical properties
were weakened.
Cell attachment studies were conducted by seeding human PDL cells on the nCS
alginate samples
and incubating for 24 h under standard conditions with MEM media. SEM studies revealed that the
cells attached to 95 nCS:5 alginate and the 90 nCS:10 alginate scaffolds but not to the 50:50 scaffolds
with the greatest attachment to the 95:5 scaffolds. Also it was observed that the cells attached to the
nCS:alginate scaffolds were spherical rather than elongated like those attached to the nCS alone scaf-
folds (
Figure 19.10
). Cell viability assays, however, revealed no significant differences among cells
cultured on the nCS:alginate scaffolds although the 95 nCS:5 alginate group did possess the highest
values of viability compared to the other two composite groups and nCS alone (
Figure 19.11
).
1
(A)
(B)
nCS:alginate (90:10)
nCS:alginate (50:50)
FIGURE 19.9
SEM images of nCS and alginate samples after setting without incubation: (A) Alginate layer wraps around the
cluster of crystals of nCS. (B) More alginate layers than separate clusters of nCS particles.
(A)
(B)
(C)
60
µ
m 500X
60
µ
m 500X
60
µ
m 500X
FIGURE 19.10
SEM images of periodontal cell attachment on nCS scaffolds after 24 h of incubation (500
). (A) 95 nCS:5
3
alginate; (B) 50 nCS:50 alginate; (C) nCS alone.
