Biomedical Engineering Reference
In-Depth Information
(A)
2
*
NT
Flat
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Day 1
Day 3
(B)
1.4
NT
Flat
1.2
1
**
0.8
*
*
0.6
0.4
0.2
0
Day 1
Day 3
FIGURE 17.3
Ratio of 5-
-2
0
-deoxyuridine (EdU) positive (A) ECs and (B) VSMCs on flat or NT substrate normalized
by the average proportion of positive cells on flat surfaces on day 1 and 3. Data are presented as
average
ethynyl
standard deviation. *
P
,
0.05, **
P
,
0.01 versus same day flat control.
Reprinted with permission from Ref.
[24]
.
6
2% to 10% (
Figure 17.4B, F, G, J, and K
). With regard to the flat surface and 25 nm NTs, because
large microscale focal adhesion can form, the cells can attach and spread well in 10%, 5%, or 2%
serum. As for the large NTs with size of 50
100 nm, because they constrain cell focal adhesion to
the top of the tube walls, high quality is needed for the small focal adhesion to support stable cell
adhesion. As shown in
Figure 17.4G, H, K, and L
), when cultured in 10% or 5% serum, the
adsorbed proteins not only widen the intertubular areas but also provide adequate integrin adhesion
sites, thus giving rise to enough high-quality focal adhesion and good cell adhesion. In 2% serum,
the smaller amount of adsorbed proteins results in narrow tube walls, a low density of integrin
adhesion sites, low-quality small focal adhesion, and poor cell adhesion (
Figure 17.4C and D
).
