Biomedical Engineering Reference
In-Depth Information
CHAPTER
14
Characterization of Silver
Nanoparticles Incorporated
Acrylic-Based Tissue Conditioner
with Antimicrobial Effect
and Cytocompatibility
Ki Young Nam
a
and Chul Jae Lee
b
a
Department of Dentistry, College of Medicine, Keimyung University, Daegu, Korea
b
School of Chemical Industry, Yeungnam College of Science & Technology, Daegu, Korea
CHAPTER OUTLINE
14.1 Introduction ............................................................................................................................... 283
14.2 Preparation and identification of silver nanoparticles................................................................... 285
14.3 Acrylic tissue conditioner combined with silver nanoparticles ...................................................... 287
14.3.1 Fabrication of Ag
-tissue conditioner composites..................................................... 287
14.3.2 In vitro antimicrobial effects on
.... 287
14.3.3 Cytotoxic test on human gingival cell line ............................................................... 289
14.4 Characterization of Ag
-tissue conditioner composites ................................................................. 289
14.4.1 Determination of eluted Ag
1
from the specimens .................................................... 289
14.4.2 Microstructure of Ag
-tissue conditioner composites................................................ 291
14.5 Conclusions............................................................................................................................... 292
References ......................................................................................................................................... 293
S. aureus, Streptococcus mutans
, and
C. albicans
14.1
Introduction
Tissue conditioners have been commonly used to enhance the recovery of denture-bearing tissues
from trauma, damage, or residual ridge resorption usually caused by ill-fitting dentures (
Figures 14.1
and 14.2
). However, these materials are degenerated and are easily degradable with time. The sur-
faces of acrylic gel polymer are susceptible to colonization by microorganisms
[1]
, and microbial
growth from the adherence of microbial cells is promoted by its rough surface and adhesive interac-
tions between Candida species and oral bacteria, mostly Candida albicans (C. albicans) and oral
