Biomedical Engineering Reference
In-Depth Information
(E)
9
8
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1
0
(A)
(B)
d
c
b
(C)
(D)
a
(G)
l
(F)
h
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FIGURE 6.7
Antibacterial activity of uncured primers in ADT. (A
D) Control primer, 10QADM, 0.05NAg, and
10QADM
0.05NAg, respectively. Note a small inhibition zone for control, and much wider inhibition zones
for primers with QADM and NAg. This example is for mutans streptococci. Total microorganisms and total
streptococci had similar results. (E
1
G) Inhibition zone data for total microorganisms, total streptococci, and
mutans streptococci, respectively. Each value is mean
6
sd (
n 5
6). Bars with dissimilar letters indicate values
that are significantly different (
P ,
0.05).
Adapted from Ref. [53] with permission.
longitudinal axis on 320-grit silicon carbide paper until the occlusal enamel was completely
removed. As shown in Figure 6.8A , the dentin surface was etched with 37% phosphoric acid gel
for 15 s and rinsed with distilled water for 15 s, following a previous study [56] . The primer was
applied with a brush-tipped applicator and rubbed in for 15 s. The solvent was removed with a
stream of air for 5 s. Then the adhesive was applied and light-cured for 10 s (Optilux VCL 401,
Demetron Kerr, Danbury, CT). A stainless-steel iris, having a central opening with a diameter of
4 mm and a thickness of 1.5 mm, was held against the adhesive-treated dentin surface. The central
opening was filled with a composite (TPH, Caulk/Dentsply, Milford, DE), and light-cured for 60 s.
The bonded specimens were stored in distilled water at 37 C for 24 h. A chisel was connected with
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