Biomedical Engineering Reference
In-Depth Information
2.5
a
a
(A)
a
b
a
b
c
2.0
bc
b
c
c
1.5
d
d
d
d
1.0
0.5
0.0
e
f
(B)
e
ef
35
ef
f
g
fg
30
g
g
25
20
h
h
15
h
h
10
5
0
j
(C)
j
i
j
i
j
i
j
18
i
i
i
16
14
12
10
8
6
4
2
0
k
k
k
k
FIGURE 6.6
Biofilm viability, growth, and acid production. (A) MTT metabolic activity, (B) CFU counts, and (C) lactic acid
production of 3-days biofilms on the composites water-aged for 1
180 days. Each value is mean
6
sd (
n
5
6).
In each plot, values with dissimilar letters are significantly different (
0.05). For the MTT assay, a higher
absorbance indicates a higher formazan concentration, which in turn indicates a higher metabolic activity in
the biofilm. NACP
P
,
QADM had biofilm metabolic activity and lactic acid that were about half of those on
commercial composites, and CFU about one-third of those on commercial composites (
P
,
0.05). Aging for
1
180 days did not reduce the antibacterial potency of the NACP
QADM nanocomposite (
P
.
0.1).
