Biomedical Engineering Reference
In-Depth Information
Figure 5.
Effect of
PMT
genes on engineered human oral mucosa structure.
C. albicans
strains were seeded onto the tissue and cultured for 22 h at 37°C, after which time biopsies
were taken, stained using Masson's trichome and observed under an optical microscope.
(Color image of this fi gure appears in the color plate section at the end of the topic.)
to several antifungal molecules (Prill et al. 2005). Thus even if
Pmt
5 gene
is expressed by
C. albicans
, its function is not detectable under standard
conditions. Interestingly, in an environment that includes an infected host,
Pmt
5 may promote the virulence of
C. albicans
. Indeed, in our engineered
human oral mucosa model, we observed defective hyphae formation by
the
Pmt
5 mutant (Rouabhia et al. 2005). This defect contradicts previously
reported data (Prill et al. 2005). It is therefore crucial that we further
investigate the mechanisms involving
Pmt
5 in each experimental condition
that leads to
C. albicans
virulence or non-virulence.
