Biomedical Engineering Reference
In-Depth Information
Table 2.
Metabolic pathway genes and primers used for the detection of toxigenic fungi.
S. No
Toxin
Target gene
Primer sequence
Tm (°C)
Amplic-on
size (bp)
Reference
Tri5
1
Trichot
h-cenes
GAGAACTTTCCCACCGAATAT
58°C
450 Bp
Venkataramana et al., 2011
GATAAGGTTCAATGAGCAGAG
Tri5
GCT GCT CAT CAC TTT GCT CAG
68°C
658 Bp
Niessen and vogel, 1998
CTG ATC TGG TCA CGC TCA TC
Tri6
GATCTAAACGACTATGAATCACC
58°C
541 Bp
Venkataramana et al., 2011
GCCTATAGTGATCTCGCATGT
TRI6
CTC TTT GAT CGT GTT GCG TC
62°C
596 bP
Bluhm et al., 2002
CTT GTG TAT CCG CCT ATA GTG ATC
2
DON
Tri13
GGC TT ACG ACT CCT CAA CAA TGG
60°C
AGA GCC CTG CGA AAG(C/T) ACT GGT GC
3
NIV
Tri7
GGC TTT ACG ACT CCT CAA CAA TGG
60°C
161 Bp
Lee et al., 2001
AGA GCC CTG CGA AAG(C/T) ACT GGT GC
Fum5
6
Fumoni
s-ins
GTC GAG TTG TTG ACC ACT GCG
62°C
845 Bp
Bluhm et al., 2002
CGT ATC GTC AGC ATG ATG TAG C
Fum1
ATTATGGGCATCTTACCTGGAT
58°C
798 Bp
Venkataramana et al., 2011
ACGCAAGCTCCTGTGACAGA
