Biomedical Engineering Reference
In-Depth Information
very low. It was speculated that the principle ability of a
F. verticillioides
isolate to produce fumonisin will depend on the presence or absence of
the
fum1
gene but additional factors may be necessary which regulate the
concentrations of fumonisin fi nally produced. Similar kind of assay for
detection of fumonisin producers was published by Bluhm et al. (2002)
and Ramana et al. (2011). These systems were based on the
fum1
and
fum13
gene sequences of
F. proliferatum
and
F. Verticillioides
and were applied
to the detection of these fungi in artifi cially contaminated cornmeal in a
multiplex PCR assay. Potential producers of fumonisins were detected
together with potential trichothecene producers (
tri5
and
tri6
genes).
Figure 13.
PCR amplifi cation
fum 13
gene of toxigenic
F. verticillioides
and
F. proliferatum
.
Lane M-1 kb DNA marker; lane 1 & 2 -
F. verticillioides
standard strains; lane 3 & 4-
F.
proliferatum
stand rd strains; Lane 5, 6, 7 & 8-
F. verticillioides
isolates; Lane 9 & 10-
F.
proliferatum
isolates, Lane 11- non toxigenic
F. verticillioides
isolate; Lane 12- non toxigenic
F. proliferatum
isolate; lane13- negative control.
Figure 14.
mPCR for the simultaneous detection of trichothecene and fumonisin
producing
Fusarium
spp. 1- 1Kb DNA marker, 2- mixed DNA, 3- trichothecene positive
F.
graminearum
4- trichothecene positive
F. culmorum
, 5- fumonisin positive
F. verticillioides
6- fumonisin positive
Fusariu
m isolate, 7- non toxic
Fusarium
isolate, 8- negative control.
