Biomedical Engineering Reference
In-Depth Information
and in cereals. Using a different concept for primer design and SYBRGreen
I as fl uorescent dye, Bu et al
.
(2005) described a quantitative real-time
PCR assay for
A. fl avus
, among other medically important fungi, in pure
cultures and in medical specimens. Haugland and Vesper (2000) published
primers for the diagnosis of a wide range of fungal species in a patent
application. No PCR-based detection assays have been described yet for
the rarely encountered afl atoxin producers
A. bombycis, A. ochraceoroseus
and
A. pseudotamari
(Bennett and Klich 2003). A multiplex PCR based
Figure 8.
Detection of afl atoxin producing
Aspergillus
species targeting
afl R
gene. Lane1-
1 kb DNA lader, lane 2-7 afl atoxigenic
Aspergillus
spp., Lane8- negative control.
Figure 9.
Detection of afl atoxin producing
Aspergillus
species targeting
nor1
gene. Lane1-
1 kb DNA lader, Lane2- negative control. lane 3-8 afl atoxigenic
Aspergillus
spp.
method was developed and evaluated for the group specifi c detection of
afl atoxigenic
Aspergillus
spp., targeting nor1 gene along with Trichothecene
and Fumonisin producing fungal spp. (Priyanaka et al. 2011 unpublished
data).
