Biomedical Engineering Reference
In-Depth Information
Figure 2.
Develop of the septoriosis glume blotch (necrotic zones size, mm) on wheat
leaves was infected the fungus
S. nodorum
strains with different aggressiveness: 1 - low
- 4VD; 2 - middle - 6VD; 3- highly - 9MN; 4- highly - Bas1.
aggressive strain 4VD. Strains 9MN and Bas1 characterized by a shorter
latent period and formation of pycnidia on leaves of wheat as early as the 7th
day of experiment. At the same time strains 6VD and 4VD formed pycnidia
on the leaves only in 12 and 14 days, respectively. Therefore strains 9MN
and Bas1 were more aggressive than strains 6VD and 4VD.
It is known that in the process of growth and development of pathogenic
fungi on the plants there are signifi cant changes in the composition of their
cell walls (El Gueddari et al. 2002). For example, chitin of cell wall of rust
pathogen infectious hyphae changes into chitosan when they penetrate
into the plant tissue. It is supposed that, this is related to the release of
fungal enzymes in the extracellular space, which are deacetylating chitin.
Interestingly, these enzymes are important in manifestation of pathogen
aggressiveness and, therefore, will help in its penetration into the plant
tissue (Deising and Siegrist 2006). In this context, we studied the effect
of culture fi ltrate of the strains of the fungus
S. nodorum
on the degree of
acetylation of chitin. It was found that the strains of the fungus with high
aggressiveness 9MN and Bas1 are deacetylating chitin more than strains
with low aggressiveness (
Figure 3).
Thus, if the degree of chitin acetylation
before the experiment was 85% after processing of culture fi ltrate of the
