Biomedical Engineering Reference
In-Depth Information
C
HAPTER
15
PCR-Diagnostic of
Septoria
nodorum
Berk. Aggresivenes
in Wheat Tissue
I.V. Maksimov* and A. Sh. Valeev
Abstract
The
Septoria
(syn.
Stagonospora
)
nodorum
(Berk.) E. Castell. & Germano [teleo-
morf:
Phaeosphaeria
(syn.
Leptosphaeria
)
nodorum
(E. Müll.) Hedjar.] is one of the
most signifi cant plant pathogen which cause leaf spot diseases of wheat and
other cereal crop. The identifi cations of
S. nodorum
in wheat plant tissues and
evaluation of pathogens aggressiveness degree by the use of PCR with primers
to chitin deacetylase gene are discussed. Differences in gene expression activity
among fungal strains dependent on the aggressiveness of the pathogen were
observed.
INTRODUCTION
The septoria glume blotch—one of the most common diseases of wheat
and its agents pose a serious threat to agriculture destroy a biological yield
on 30-40% (Eyal 1999). The fungus
Septoria
(syn.
Stagonospora
)
nodorum
(Berk.) E. Castell. & Germano [teleomorf:
Phaeosphaeria
(syn.
Leptosphaeria
)
nodorum
(E. Müll.) Hedjar.] is actively developing on wheat and its visual
symptoms in seeds are absent, but in plants the disease appears in the
form of necrosis, desiccation and pycnidia are formed in the infected parts
of the plants (Eyal 1999, Solomon et al. 2006). This pathogen should not
be confused with Septoria leaf blotch, caused by
Septoria tritici
Rob et
