Biomedical Engineering Reference
In-Depth Information
Table 3.
List of genes of
Magnaporthe oryzae
identifi ed and characterized in studies
using ATMT.
Gene name
Function or domain
Key phenotypes
References
EXP5
Karyopherins
Reduced pathogenicity
on leaf and no root
colonization
Tucker et al. 2010
MoLDB1
LIM binding domain
Impaired sexual and
asexual reproduction,
reduced pathogenicity
Li et al. 2010
MoRIC8
Regulator of GTP-
binding protein
No pathogenicity
Li et al. 2010
COM1
Putative transcription
factor with helix loop
helix
Altered conidial
morphology,
reduced pathogenicity
Yang et al. 2010
COS1
Zinc-fi nger protein
Defective in conidiation
Zhou et al. 2009
DES1
Unknown
Reduced pathogenicity
Chi et al. 2009
ABC4
ABC transporter
No pathogenicity
Gupta and Chattoo
2008
MCK1
MAP kinase kinase
kinase
No pathogenicity
Jeon et al. 2008
RGS1
RGS1-containing protein
No pathogenicity
Liu et al. 2007
MGA1
Unknown
No pathogenicity on
both leaf and root
Gupta and Chattoo
2007
ABC3
Multidrug resistance
transporter
No pathogenicity
Sun et al. 2006
HEX1
Hexagonal peroxisome
protein
Reduced pathogenicity
Soundararajan et al.
2004
complex trait that is governed by diverse cellular processes. Expectation is
that with large-scale mutant libraries, the number of novel pathogenicity
genes identifi ed via ATMT will increase dramatically in the years to come,
revealing intriguing aspects of molecular mechanisms that enable the fungus
to cause disease on plants.
Signifi cant progress has been achieved in the identifi cation of genes
in
M. oryzae
by generating a large library of mutants and functional
characterization of unprecedented number of pathogenicity genes. This
systematic approach has rendered
M. oryzae
as a model for many fungi.
Jeon et al. (2007) used ATMT technique and generated a total of 21,070
insertional mutants in the rice blast fungus,
M. oryzae
. Using the high-
throughput phenotype screening pipeline, they have identifi ed 202 new
pathogenicity loci distributed genome-wide. These genes are involved in
