Biomedical Engineering Reference
In-Depth Information
Figure 2.
Disease cycle of rice blast fungus,
Magnaporthe oryzae.
(Color image of this fi gure appears in the color plate section at the end of the topic.)
to saturate the genome of
M. oryzae
with mutations and identifi ed several
new pathogenicity genes for this fungus. Thus, ATMT is an ideal tool to
create large-scale insertional mutagenesis and for genome-wide mutational
analysis in fungi (Jeon et al. 2007).
In this chapter, we will report an overview of the pathogenicity genes
identifi ed using ATMT technique for two phytopathogens,
M. oryzae
and
V. dahliae
. The total number of pathogenicity genes described below may
not be comprehensive, since new genes identifi ed and characterized are
currently in publications being prepared.
METHODS
Principles of
Agrobacterium tumefaciens
-Mediated
Transformation
The gram-negative, soil inhabiting
A. tumefaciens
is a plant pathogen
capable of producing tumors in plants. Tumor development results from
an integration of a portion of
A. tumefaciens
transfer-DNA (T-DNA), which
is located in tumor-inducing (Ti) plasmid, into the plant cell genome by
