Biomedical Engineering Reference
In-Depth Information
throughput for simultaneous detection of multiple targets could be
achieved if various gold nanorods with different LPWs are carefully
designed and then assembled on chips. In 2009, Huang and coworkers
demonstrated the use of ive kinds of gold nanorods to construct
the multi-throughput LSPR biosensor for simultaneous detection of
antigen-antibody interactions in the wavelength range from 530 to
940 nm.
70
Thus, the broad tunability of the LPW of gold nanorods
and other NMNPs open a door for multiplex LSPR biosensing.
4.5
Conclusions and Outlook
In conclusion, LSPR sensing offers many promising features and
potential applications (Table 4.1) and has been demonstrated
by the development of LSPR-based biosensors in this chapter.
However, there are still many challenges before they can be used
in practice. These include the improvements in the detection
capability, sensitivity
71,72
or combination with other identiication
techniques.
73
A combined multidisciplinary research effort is still
necessary to collect all these features to develop highly sensitive and
reliable biosensing platforms.
Table 4.1
Summary of the LSPR-based biosensing assays with various
nanostructures
Nanostructure
Target
Au nanospheres
Streptavidin
17,23
Anti-BSA
24
or anti-HSA
25
Au or Ag nanospheres
Various anti-IgG
39,40
Au nanorods
Neutravidin
28
Au nanoring
Au nanohole
Neutravidin
54,55
Anti-biotin,
44
ConA,
45
anti-ADDL,
46
anti-ADDL (sandwich)
47
Ag triangles
Nucleic acids,
50
anti-IgA, IgD, IgG,
IgM,
52
C-reactive protein and
ibrinogen
69
Au-capped nanoparticles layer
substrate
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