Colorimetric Bioassay Using Noble Metal Nanoparticles - From Bioimaging to Biosensors: Noble Metal Nanoparticles in Biodetection - page 29

Chapter 3
Colorimetric Bioassay Using Noble
Metal Nanoparticles
Ming-Feng Huang a , Yang-Wei Lin b , and Huan-Tsung Chang a, *
a Department of Chemistry, National Taiwan University,
1, Section 4, Roosevelt Road, Taipei, Taiwan
b Department of Chemistry, National Changhua University of Education,
1, Jin-De Road, Changhua City 500, Taiwan
* [e-mail]
In this chapter, we attempt to present the development and
applications of metal nanoparticle-based colorimetric assays.
The sensing strategies are based on analyte-induced nanoparticle
(NP) aggregation and dispersion. We begin with the preparation,
stabilization, and surface functionalization of Au and Ag NPs. A
general introduction of the optical properties of Au and Ag NPs
briely follows. After providing a general background, we concentrate
on their applications of sensing analytes, including DNA, proteins,
and small solutes, through interparticle crosslinking and non-
crosslinking aggregation events.
Edited by Lai-Kwan Chau and Huan-Tsung Chang
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