Biomedical Engineering Reference
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selective and concentrating probes, the analysis of glutathione in red
blood cells and of cysteine in plasma has been accomplished. Chang
et al
. described the use of aptamer-modiied AuNPs as selective
probe and bare AuNPs as LDI matrices for the extraction of anti-
aptamer corresponding to adenosine triphosphate.
33
Figure 12.2
summarizes this two-step sample preparation for the analysis
of adenosine triphosphate by LDI-MS. The LOD of adenosine
triphosphate obtained by this approach is 0.48 μM. They suggested
that this method can be applied for monitoring the bioactivity of
cells through determinations of the concentrations of adenosine
triphosphate and glutathione.
Figure 12.2
Illustration of the use of aptamer-AuNPs for selective
extraction of adenosine triphosphate that was then detected
using bare AuNPs as LDI matrices. Reprinted with permission
from Ref. 33.
Self-assembled multilayer ilms of citrate-AuNPs could be utilized
to extract environmental pollutants dimethyldistearylammonium
chloride and pyrene from diluted solutions (concentrations less than
10
−10
g/L).
23
The integration of magnetic NPs and citrate-AuNPs could
selectively trap positively charged proteins and peptides through
electrostatic interaction.
34
The analytes adsorbed on the NP surface
could be further collected using magnetic ield and then detected
using organic matrices. The selectivity of this kind of NPs is highly
dependent of solution pH. The enrichment of Met- and Leu-enkephalin
peptides have been accomplished through the combination of AuNP-
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