Biomedical Engineering Reference
In-Depth Information
Chapter 12
Mass Spectrometric Bioanalysis Assisted
by Noble Metal Nanoparticles
Yen-Hsiu Lin and Wei-Lung Tseng
*
Department of Chemistry, National Sun Yat-sen University,
Lien-hai Road, Kaohsiung, Taiwan
*
tsengwl@mail.nsysu.edu.tw
The chapter reviews recent developments in the application of
noble metal nanoparticles (NPs) to laser desorption/ionization
mass spectrometry (LDI-MS), secondary ion MS (SIMS), and
inductively coupled plasma MS (ICP-MS). Metal NPs as matrices in
LDI-MS enable the selective and sensitive detection of biomolecules
such as proteins, peptides, aminothiols, drugs, and carbohydrates.
Metal NPs act as both signal enhancers and target concentrators in
SIMS when they are attached to a well-controlled surface such as
self-assembled monolayers. ICP-MS is well suited to determine the
concentration of metal NPs that have been conjugated to antibody.
Besides, metal NPs are capable of serving as afinity and concentrating
probes for target analytes prior to LDI-MS. A table summarizes the
applications of metal NPs to the analysis of a variety of analytes in
LDI-MS.
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