Biomedical Engineering Reference
In-Depth Information
Chapter 7
Microfluidics for Biosensor Chips
Shau-Chun Wang
Department of Chemistry and Biochemistry, National Chung Cheng University,
168 University Road, Ming-Hsiung, Chia-Yi 621, Taiwan
chescw@ccu.edu.tw
Microluidic devices that integrate functional units to perform bio-
analytical and diagnostic tests in chips have some unique advantages,
such as reducing reagent and sample consumption, and avoiding
contamination during sample transfer between analysis procedures.
This chapter reviews biosensing techniques that utilize noble metal
nanoparticles to develop various spectroscopic methods, including
label-free scattering and luorophore-tagged emission methods.
Unlike bulk materials such as mirror-like metallic ilms, nano-
particles of noble metals, such as gold (Au) and silver (Ag), absorb
and scatter the incident light to appear colors, known as localized
surface plasmon resonance (LSPR) and also known as particle
plasmon resonance (PPR). The resonance frequency of the collective
oscillating electrons is dependent on the dielectric properties of
the nanoparticles and their surrounding medium. In other words,
the binding events of immobilized probes on the particle surfaces
will change the scattering frequency sensitively. Also the surface
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