Biomedical Engineering Reference
In-Depth Information
FIGURE 3.9
Unmodiied. AuNPs'. colorimetric. detection.. A. primer. targeting. HCV. RNA. (short. strands). is. mixed. with.
extracted.RNA.(long.strands).in.a.hybridization.buffer.solution.containing.NaCl..If.the.extracted.RNA.is.HCV.
RNA,.hybridization.occurs..Upon.addition.of.colloidal.AuNPs.to.the.solution.with.non-hybridized.primer,.the.
primer.adsorbs.onto.the.AuNPs,.protecting.them.from.aggregation,.and.the.color.remains.red.(bottom-right.
tube).. Upon. addition. of. AuNPs. to. the. solution. where. hybridization. occurred,. the. AuNPs. aggregate,. and. the.
color.changes.to.blue.(bottom-left.tube).
