Biomedical Engineering Reference
In-Depth Information
(a)
Gold-coated NP layer formed area
1 cm
Anti-IgA
antibody
Anti-IgD
antibody
Anti-IgG
antibody
Anti-IgM
antibody
Anti-CRP
antibody
Anti-fibrinogen
antibody
IgA
(b)
0.12
1 mg ml
-1
100 µg ml
-1
1 µg ml
-1
100 ng ml
-1
1 ng ml
-1
100 pg ml
-1
1 pg ml
-1
100 fg ml
-1
1 fg ml
-1
Bank (PBS buffer)
0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
(c)
0.15
0.10
0.05
0
Anti-IgA
Anti-IgD
1 mg ml
-1
Anti-IgG
Antigen
concentration
Anti-IgM
Anti-CRP
Anti-fibrinogen
0 g
COLOR FIGURE 5.7
LSPR-based.nanochips.for.multiplexed.sensing.of,.e.g.,.immunoglobulin.A.(IgA)..(a).Photograph.of.the.LSPR-
based.nanochip.(20.mm.×.60.mm)..The.nanochip.structure.consists.of.a.lat.gold.ilm,.self-assembled.monolayer.
of.silica.nanospheres,.and.a.gold.coating.over.the.nanospheres..(b.and.c).Absorbance.measurements.at.each.spot.
of.the.multiarray.nanochip.for.monitoring.the.binding.of.IgA.to.six.different.types.of.antibodies..The.antibodies.
were.immobilized.on.the.chip.by.the.nanoliter.dispensing.system.and.result.in.a.total.of.300.spots.separated.by.
1.mm.to.prevent.cross-contamination..Various.concentrations.of.antigens.were.incubated.for.30.min.and.LSPR.
absorption.spectra.were.then.taken.with.a.iber-coupled.ultraviolet-visible.spectrometer.in.a.relection.conigu-
ration..Detection.was.linear.up.to.1.μi.ml
−ml
with.a.limit.of.detection.of.100.pg.ml
−i
.for.all.of.the.proteins..(©.2006.
American.Chemical.Society..Used.with.permission.)
