Biomedical Engineering Reference
In-Depth Information
(a)
a
b
c
4 µm
10 µm
10 µm
Effective Shell ‰ickness (nm)
(b)
0
5
10
15
20
1.2
Au-ph-DNA
Au-ph-DNA
2.2
0
5
10
15
20
2 30
35
40
45
50
55
60
65
+ Kpn I
1.0
+ Sal I
+ Kpn I
2.1
+ Xho I
+ Hind III
0.8
2.0
Au-ph
+ Sal I
1.9
0.6
+ Xho I
1.8
0.4
+ Hind III
1.7
0.2
Au-ph
1.6
0.0
500
550
600
650
700
0
10
20
30
40
50
60
Wavelength (nm)
Base Pairs Remaining on Ruler
COLOR FIGURE 5.6
Nanoplasmonic.molecular.rulers.for.measuring.nuclease.activity..(a).Dark.ield.scattering.images:.a,.single-target.
single.nanoparticle.isolated.from.other.Au.or.Au-DNA.NPs.for.spectroscopic.examination;.b,.c,.true-color.images.
of.single.Au.NP.(b).(540.nm.peak).and.Au-DNA.NP.(c).(607.nm.peak)..(b).Typical.LSPR.scattering.spectra.and.the.
corresponding.maximum.scattering.wavelengths.( λ max ).of.the.Au-DNA.nanoconjugates.after.cleavage.reactions.
with.four.endonucleases.( Hind III,. Xho I,. Sal I,.and. Kpn I).and.as.a.function.of.the.number.of.base.pairs.remain-
ing.attached.to.the.Au.nanoparticle.after.the.cleavage..The.red.curve.is.a.it.from.a.semiempirical.model.using.
a.Langevin-type.dependence.of.the.refractive.index.vs..dsDNA.length..The.error.bars.represent.the.standard.
deviation.in.the.measurement.of.20.nanoparticles..(©.2006.Macmillan.Publisher's.Ltd..Used.with.permission.)
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