Biomedical Engineering Reference
In-Depth Information
17
ProductionofBareMultiple-Element
MagneticNanoparticlesandTheirUsein
FastDetectionandRemovalofPathogenic
Bacteria
fr
omWaterResources
KhaledN.Elshuraydeh,HananI.Malkawi,andMonaHassuneh
HigherCouncilforScienceandTechnology,DepartmentofBiological
Sciences,YarmoukUniversity,Irbid,Jordan
CONTENTS
17.1. Introduction.and.Background........................................................................................ 332
17.2. Materials.and.Methods.................................................................................................... 333
17.2.1. Preparation.of.Multiple-Element.Magnetic.Nanoparticles.(MMNs).......... 333
17.2.2. Characterization.of.the.MMNs.Using.Atomic.Absorption.......................... 333
17.2.3. Preparation.of.MMNs.for.Examination.and.Imaging.under.
Transmission.Electron.Microscope.(TEM)..................................................... 334
17.2.4. Preparation.of.MMNs.for.Examination.under.Scanning.Electron.
Microscope.(SEM)............................................................................................... 334
17.2.5. Examining.the.Effect.(Antibacterial.Activity).of.MMNs.on.Bacterial.
Growth................................................................................................................. 334
17.2.6. Preparation.and.Puriication.of.Bacterial.Suspension.for.Examination.
and.Imaging.under.Transmission.Electron.Microscope.(TEM).................. 335
17.2.7. Capturing.and/or.Binding.of.Bacterial.Cells.with.Unconjugated.
(Bare).MMNs....................................................................................................... 335
17.2.8. Use.of.MMN.Preparations.to.Detect.Water.Samples.Artiicially.
Contaminated.with.Bacteria............................................................................. 337
17.2.9. Treatment.of.Wastewater:.Using.MMNs.for.Detection,.Enumeration,.
and.Removal.of.Bacterial.Contamination....................................................... 338
17.2.10. Use.of.MMNs.for.Detection,.Enumeration,.and.Removal.of.Bacterial.
Contamination.in.Grey.Water.......................................................................... 339
17.2.11. Transmission.Electron.Microscopy.Sectioning.and.Imaging...................... 340
17.3. Results.and.Discussion.................................................................................................... 341
References..................................................................................................................................... 342
331
