Biomedical Engineering Reference
In-Depth Information
2
Bionanote
chnologyStrategiesforCellDetection
NasserAghdamiandSarahRanjbarVaziri
DepartmentofRegenerativeMedicineforStemCellBiologyand
Technology,RoyanInstitute,ACECR,Tehran,Iran
CONTENTS
2.1. Introduction......................................................................................................................... 11
2.2. Cell.Tracking.Agents.......................................................................................................... 14
2.3. Fluorescence........................................................................................................................ 15
2.4. Quantum.Dots.................................................................................................................... 17
2.5. Synthesis,.Solubilization,.and.Bioconjugation............................................................... 19
2.6. Extracellular.and.Intracellular.Targeting....................................................................... 20
2.7. In.Vivo.Application.of.Quantum.Dots............................................................................ 22
2.8. Cellular.Fate.Tracking.in.Developmental.Biology......................................................... 23
2.9. Cell.Tracking.in.Stem.Cell.Therapy................................................................................. 24
2.10. Using.Quantum.Dots.as.Optical.Contrast.Agents........................................................ 25
2.11. Tumor.Targeting.and.Imaging......................................................................................... 25
2.12. Lymph.Node.and.Vascular.Mapping.............................................................................. 26
2.13. Bimodal.Molecular.Imaging.Probes................................................................................ 28
2.14. Cytotoxicity......................................................................................................................... 29
2.15. Magnetic.Resonance.Imaging.......................................................................................... 31
2.16. Superparamagnetic.Nanoparticles.................................................................................. 34
2.17. SPIO.Applications.in.Cell.Tracking................................................................................. 37
2.18. Limitations........................................................................................................................... 39
References....................................................................................................................................... 39
2.1 Introduction
Every.time.we.start.to.read.about.new.technologies.or.new.sciences,.we.can.ind.some-
thing. relevant. from. ancient. mythologies. of. different. civilizations.. It. seems. that. human.
beings.live.in.a.closed.historical.cycle;.at.least.our.thinking.or.ideas.remain.unchangeable.
in.history,.and.in.this.long.story,.science.just.helps.us.to.bring.ideas.and.imagination.to.
practical.realities.and.applications..A.hero.who.never.dies,.and.after.any.injury.his.body.
regenerates. the. lesion,. is. a. classic. example. to. think. about. the. regenerative. potential. of.
stem.cells..In.ancient.Iranian.mythology,.Esfandiyar.was.such.a.heroic.character;.he.would.
recover.from.his.wounds.after.every.encounter.with.his.enemies.[Ferdowsi,.Shahnameh,.
977.A.D]..Nowadays.we.live.in.a.faster.world,.where.there.are.more.accidents.that.lead.to.
loss.of.organs.or.functions,.and.anyone.can.aspire.to.have.this.power.to.regenerate.lost.
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