Biomedical Engineering Reference
In-Depth Information
PS Unshrunk
PS Shrunk
SEM: PS Shrunk
A
5 mm
1 mm
10 mm
B
PO Unshrunk
PS Shrunk
SEM: PO Shrunk
1 mm
5 mm
10 mm
C
D
Channel
Cross-Section
1
1
0.5
0.5
0
0
0
0
0.3
Distance (mm)
0.6
0.15
Distance (mm)
0.3
150 µm
E
% Change in
Horizontal Line
Width
45.00
% Change in
Vertical Line
Width
40.82
Shrunk Line
Height (µm)
% Change in
Area
% Change in
X
% Change in
Y
Average
94.87
77.43
77.29
47.54
Standard
Deviation
0.51
1.10
1.88
8.82
9.66
11.70
FIGURE 13.9
Characterization.of.thermoplastic.shrinkage..It.is.apparent.that.the.shrinkage.of.PO.(B).is.much.greater.than.
that.of.PS.(A)..The.resulting.enhancement.in.shrinkage.of.PO.allowed.for.an.improved.aspect.ratio,.as.shown.in.
the.SEM.of.the.PO.surface.vs..that.of.PS..Postshrinkage,.these.patterned.features.can.be.used.for.the.fabrication.
of.microluidic.channels.(C).with.rounded.channel.shapes.(D)..The.average.surface.area.reduction.of.PO.was.
approximately.95%.in.both.the.x.and.y.directions.(E).
Due.to.the.heat-induced.shrinking.of.the.polymer,.the.protein.arrays.are.pulled.together,.
resulting.in.increased.array.density.as.well.as.antibody.concentration..Thus,.to.fully.uti-
lize.the.size.reduction.of.PO.ilms,.luorescently.tagged.polyclonal.antibodies.were.depos-
ited. onto. PO. ilms. using. standard. µCP,. whereby. PDMS. stamps. containing. the. proteins.
were.stamped.onto.the.PO.surface..To.facilitate.the.uniform.shrinking.of.the.array,.the.PO.
