Biomedical Engineering Reference
In-Depth Information
(a)
PDMS
Poly(TMSMA-r-PEGMA)
Substrate
(i) Removal of PDMS
(ii) Washing with MeOH
(iii) Curing at 110°C
(i) Removal of PDMS
(ii) Curing at 110°C
Polymer
microstructure
Polymer
monolayer
O
O
O O
O
O
Si
O
O
O O
O
Si
O
O
O
O
O
O
O O O
O
O
O
Si
Si
Si
Si
O
O
O
O O O
O O O
O
O
O
PEG-OCH 3
Si
O
O
Crosslinked polymer
Polymeric monolayer
(b)
a
PM
Day 1
Day 4
b
5 mg/mL
Day 1
Day 4
c
50 mg/mL
Day 1
Day 4
d 100 mg/mL
Day 1
Day 4
FIGURE 12.2
Micropatterning. surfaces. using. a. capillary. force. lithography. approach.. (a). PDMS. mold. is. placed. on. a. spin-
coated.polymeric.surface.to.induce.a.capillary.force.and.selectively.form.patterned.microstructures.or.polymeric.
monolayers.. (b). Cell. patterns. on. microstructures. showed. more. stability. than. cells. in. polymeric. monolayers.
after.4.days.of.culture..Adapted.with.permission.from.reference.27..Copyright.2003.Wiley-VHC.Verlag.GmbH.
&.Co..KGaA.
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