Biomedical Engineering Reference
In-Depth Information
(a)
Serum Albumin
Cross-linking
with EGDE
cr-Alb coated surface
Casting cross-linked
albumin solution
Patterned Albumin Substrate
Photomask
UV light
(i)
(iii)
(b)
(ii)
FIGURE 12.1
(a).Selective.surface.modiication.on.albumin.using.UV.exposure..(b).The.selective.surface.patterning.was.used.
to. pattern. mouse. ibroblast. cells. on. an. albumin. coated. substrate.. Different. shapes. (circle,. rectangle,. and. tri-
angle).and.culture.time.(2.h.-.5.days).were.tested.to.demonstrate.the.feasibility..Adapted.with.permission.from.
reference.24..Copyright.2008.American.Chemical.Society.
makes. negative. mold. structures. without. any. transfer. procedures. employed. by. conven-
tional.µCP..It.was.shown.in.this.experiment.that.3T3.cells.in.microstructures.had.better.
stability.than.the.cells.in.polymeric.ilms..Also,.PDMS.stamps.can.be.used.to.print.living.
microbes. on. an. agarose. gel.
28
. A. monolayer. of.
E. coli
. prepared. on. the. surface. of. agarose.
gel. was. transferred. to. another. agarose. substrate. using. a. PDMS. stamp. without. damage.
by. exposure. to. air.. During. the. process,. the. agarose. concentration. for. both. inkpad. and.
substrate.is.crucial.for.generating.patterns..It.was.found.that.the.agarose.concentrations.
of.3%.and.4%.were.appropriate.for.the.inkpad.and.substrate,.respectively..Coq.et.al..stud-
ied. a. self-supporting. hydrogel. stamp. that. has. a. high. water/buffer. holding. capacity. for.
the.hosting.of.sensitive.biological.molecules.
29
.An.optimized.hydrogel.concentration.(10%.
water,.18%.PEGDA,.and.72%.HEA.prepolymer.solution).in.terms.of.rigidity.and.high.water.
absorption.made.it.possible.to.transfer.antibodies.on.various.surfaces,.including.bare.Au,.
with.minimal.damage.under.ambient.conditions..Recently,.other.researchers.have.studied.
