Nanotechnology in Biomaterials: Nanofibers in Tissue Engineering - Bionanotechnology: Global Prospects - page 218

(A) (B)

FIGURE 10.3
(See.color.insert.).Cross-sectional.(X-axis.~.60° . tilted) . CLSM.images.of.FITC-Phalloidin.and.propidium.iodide-
stained.WJ.MSCs.on.a.single,.electrospun,.aligned.iber.mat.of.PHBV:P(L-D,L)LA:PGS.(poly(glycerol.sebacate)).
(100×). showing. (A). F-actin. and. (B). nuclear. alignment. (two-sided. arrow,. direction. of. aligned. iber. axis).. Top.
images.are.the.magniied.versions.of.insets.

Studies. carried. out. with. aligned. iber. mats. with. iber. diameters. ranging. from. 0.25. to.
3.00.μm.show.that.cells.grown.on.them.align.equally.well.along.the.direction.of.the.ibers.
(Evans.et.al.,.2003;.Yost.et.al.,.2004;.Zong.et.al.,.2005;.Rockwood.et.al.,.2008;.Kenar.et.al.,.
2010)..The.single.study.carried.out.on.cardiomyocyte.function.on.electrospun.random.and.
aligned.polymer.ibers.showed.that.cellular.alignment.enhances.cardiomyocyte.matura-
tion;. cells. grown. on. aligned. electrospun. polyurethanes. had. signiicantly. lower. steady-
state.levels.of.atrial.natriuretic.peptide.(ANP).and,.as.a.result,.released.less.ANP.over.time.
than.cardiomyocytes.on.random.ibers.(Rockwood.et.al.,.2008).
In. conclusion,. nanoibers. are. novel. scaffold. materials. for. tissue. engineering,. and. the.
accumulating.data.show.them.to.be.superior.to.most.other.designs.due.to.the.versatility.
of. the. approach.. They. are. especially. suited. to. the. highly. organized. nature. of. biological.
tissues..With.developments.in.the.processing.systems,.they.will.become.the.major.scaffold.
type.

References
Agarwal.S,.Wendorff.JH,.Greiner.A..2009..Progress.in.the.ield.of.electrospinning.for.tissue.engineer-
ing.applications.. Adv Mater .21:.3343-3351.
Akhyari. P,. Fedak. PW,. Weisel. RD,. Lee. TY,. Verma. S,. Mickle. DA,. Li. RK.. 2002.. Mechanical. stretch.
regimen.enhances.the.formation.of.bioengineered.autologous.cardiac.muscle.grafts.. Circulation
106:.I137-I142.
Ashammakhi.N,.Ndreu.A,.Nikkola.L,.Wimpenny.I,.Yang.Y..2008..Advancing.tissue.engineering.by.
using.electrospun.nanoibers.. Regen Med .3:.547-574.