Biomedical Engineering Reference
In-Depth Information
Lactide
monomer
TPGS
TPGS
DCC, glutaric acid, DMSO
Stannous octoate (145°C)
PLA-TPGS
TPGS-COOH
N-Boc ethylenediamine
Trastuzumab
NHS/EDC
PLA-TPGS
Trastuzumab
TPGS-COOH
FIGURE 8.2
Schematic.illustration.for.preparation.of.the.trastuzumab-functionalized,.docetaxel-loaded.PLA-TPGS/TPGS-
COOH.nanoparticles..(Reproduced.with.permission.from.Sun.and.Feng,.
Nanomedicine
4.(4):.431-445,.2009.)
centrifuged,.washed.with.DI.water,.and.dried.under.reduced.pressure..Hereinafter,.the.
docetaxel-loaded,. trastuzumab-conjugated. NPs. of. 10,. 20,. and. 33.3%. TPGS-COOH. are.
termed. NP10-HER,. NP20-HER,. and. NP33-HER,. respectively.. The. detailed. scheme. for.
preparation.of.nanoparticles.is.shown.in.Figure 8.2.
Coumarin-6-loaded.PLA-TPGS/TPGS-COOH.NPs.with.and.without.trastuzumab.con-
jugation.were.prepared.in.the.same.way.as.the.docetaxel-loaded.NPs,.except.the.drug.was.
replaced.by.0.05%.(w/v).coumarin-6..Coumarin-6-loaded.NPs.were.used.for.the.in.vitro
cellular.uptake.experiment.
8.5 CharacterizationofTrastuzumab-ConjugatedNanoparticles
8.5.1 Size and Size Distribution
Particle.size,.polydispersity,.zeta.potential,.and.drug.encapsulation.eficiency.of.the.doc-
etaxel-loaded.PLA-TPGS/TPGS-COOH.NPs.of.0,.10,.20,.and.33.3%.TPGS-COOH.with.or.
without. trastuzumab. surface. modiication. are. listed. in. Table 8.1,. from. which. it. can. be.
seen.that.the.NPs.of.all.formulations.in.this.study.were.around.300.nm.in.diameter,.with.
a.polydispersity.of.around.0.15..Surface.modiication.of.the.NPs.by.trastuzumab.slightly.
increased.the.particle.size.and.polydispersity,.but.this.did.not.seem.to.be.affected.by.the.
amount.of.trastuzumab.on.the.NP.surface..From.our.point.of.view,.NPs.of.around.200 nm.
