Biology Reference
In-Depth Information
Table 4.1
Some examples of recovery rates in percent ± SD for different filtration
setup and elution conditions for various microorganisms—cont'd
Filtration
technique
Elution
technique
Water type/
volume
Surrogate
viruses
1
Filter
Elution buffer
Seeding level
1
NanoCeram
Elution
1.0% NaPP, 0.05 M
glycine (Re-
concentration:
Centricon Plus-70)
AdV:
MS2: 10
4
and
10
8
pfu
PV:
EV:
CV:
Tap/20 L
MS2: 45 ± 15
56 ± 9
Elution
3% beef extract,
0.1 M glycine pH 9.5
AdV: 10
9
PCR
units
Qβ: 1 0
12
PCR
units
Sea/40 L
Qβ: 96 ± 52
Source/40 L
Qβ: 34 ± 16
Finished/40 L
Qβ: 36 ± 20
Elution
1.5% beef extract
pH 9.75 (Re-
concentration:
Flocculation in 0.1%
FeCI
3
followed by
centrifugation)
EV: 10
2
IU
AdV: 10
3
IU
NoV GII:
10
6
GC
Pure/10 L
Tap/10 L
Raw/10 L
Elution
1.5% beef extract,
0.05 M glycine pH 9.5
(Re-concentration:
Celite elution)
NoV: 10
6
MPN
RT-PCR
units
River/
10-100 L
Tap/
10-100 L
Glass-cellu-
lose filter,
Zeta plus
Virosorp,
1MDS
Elution
1.5% beef extract
0.05 M glycine
buffer, pH 9.5 (Re-
concentration: Celite
adsorption/elution)
NoV: 10
6
MPN
RT-PCR
units
River/
10-100 L
Tap/
10-100 L
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