Molecular Methods for the Detection of Waterborne Pathogens - Waterborne Pathogens: Detection Methods and Applications - page 239

Figure 8.10 Example of microarray analysis of nine pathogens. Panels a through l
show the pattern obtained with each of the individual pathogens and panel m shows
the pattern obtained when all nine are present. (a) Aeromonas hydrophila ; (b) Klebsiella
pneumoniae ; (c) Legionella pneumophila ; (d) Pseudomonas aeruginosa ; (e) Salmonella ;
(f ) Shigella ; (g) Staphylococcus aureus ; (h) Vibrio cholerae ; (i) Vibrio parahaemolyticus ;
(j) Yersinia enterocolitica ; (k) Leptospira interrogans ; (l) Legionella bozemanii ; (m) A.
hydrophila , P. aeruginosa , Shigella , S. aureus and L. pneumophila . Source: Adapted from
Ref. 86 .
ones such as 16S rRNA, 18S rRNA, and 23S rRNA 51,68,87 to those that can
discriminate between different strains of a species. 86,88
8.2.3.7. Molecular beacons
Molecular beacons (MBs) are short singled-stranded nucleic acid
sequences (30-50 bases) that are designed to have a unique sequence
flanked by indirect repeats so that a stem-loop structure is formed. Each
end of the structure is labeled, one with a fluorophore (5′) and the other
(3′) with a universal quencher. When the stem and loop is formed, the
quencher masks the fluorescence. However, if the unique sequence
hybridizes to a complementary (target) sequence, the stem and loop