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Figure 7.14 Representative (a) f shifts and (b) D shifts as a function of E. coli O157:H7
injection time for various cell concentrations (solid lines). The dashed line shows (a) f
shifts and (b) D shifts as a function of E. coli O157:H7 (10 8 cells mL −1 ) injection time for a
sensor crystal prepared without antibody. Source: Figure 3 from Ref. 33 . Reproduced with
permission.
Figure 7.15 Resonant frequency response of PEMC-b sensor to the binding of EC
O157:H7 at concentration of 1 EC mL −1 (total sample volume was 1 L) for the dominant
resonant modes investigated. The control experiments were an anti-EC PEMC-b sensor
exposed to only phosphate buffered saline (PBS) solution in the same fashion as the
EC sample. Source: Figure 5 from Ref. 40 . Reprinted with permission. Copyright (2007)
American Chemical Society. (For color version of this figure, the reader is referred to the
online version of this topic.)
ME systems have also been developed with different recognition strate-
gies. First, in 2003, an alkaline phosphatase-labeled anti- E. coli O157:H7
antibody was immobilized on the sensor surface, with the mass change
associated with the antibody-antigen binding reaction amplified by biocat-
alytic precipitation of 5-bromo-4-chloro-3-indolyl phosphate ( Fig. 7.16 ). 42
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