Biology Reference
In-Depth Information
Figure 7.6 (a) Left image: Schematic of the flow through amperometric electrode
set-up. Source: Reproduced from Fig 1 from Ref. 97 . Right images: Fig 2 from Ref. 97 . (b)
Schematic of the LAPS set-up. Fig 1 from Ref. 98 .
Amperometric biosensors operate with a constant potential applied
between a working electrode and a reference electrode ( Fig. 7.6(a) ). On
interaction of the target analyte with the working electrode surface a redox
reaction occurs, generating a current that is proportional to the concentra-
tion of the electroactive target. Since picoampere current measurements are
possible, ultrasensitive amperometric biosensors can be obtained. 30 This is
the most widely used electrochemical technique, particularly for microbial
detection. 30
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