Optical Detection Technologies for Waterborne Pathogens - Waterborne Pathogens: Detection Methods and Applications - page 123

Table 5.2 Summary of the application of flow cytometry to waterborne pathogen monitoring
Pathogen

Detection approach LOD Sample processing Reference

Marine viruses Stained with nuclei acid

dye SYBR green-I

Not reported Small samples taken (1.5 mL),

fixed and frozen

26

Rotavirus Infection of cell lines,

followed by fluorescein
labeling

2-20 Concentrated from 500 L with

MK II filters, eluted and
organic flocculation to 50 mL

24

Bacteria (indigenous
aquifer bacterium)

CFDA/SE stained,
fluorescent microsphere
used for calibration

1 × 10 3 1 mL from of 35 mLoriginal

sample

29

Legionella FITC-labeled monoclonal

antibody

10 2 -10 3 cfu L −1 Centrifugation to concentrate,

IMS to isolate

27

Cryptosporidium Oocyst wall fluorescently

immunostained

Not confirmed but
20 oocysts L −1
detected

100 mL of wastewater concentrated
by calcium carbonate flocculation
and then centrifugation

25,28

Giardia Cyst wall fluorescently

immunostained

Not confirmed but
40 oocysts L −1
detected

100 mL of wastewater concentrated
by calcium carbonate flocculation
and then centrifugation

21,25