Optical Detection Technologies for Waterborne Pathogens - Waterborne Pathogens: Detection Methods and Applications - page 121

Figure 5.6 Schematic of flow cytometry setup. Source: Reproduced from Figure 2 from
Ref. 23 . (For color version of this figure, the reader is referred to the online version of this
topic.)
Figure 5.7 Example of a flow cytometry result. Detection of bacteria in milk using
flow cytometry. Samples were stained with SYTO BC and analyzed using a FACSCalibur
instrument. (a) Raw milk; (b) Bacteria in phosphate buffered saline; (c) Raw milk cleared
of lipids only; (d) Raw milk cleared of proteins and lipids. FL1 wavelength range was
515-565 nm. Source: Data were provided by T. Gunasekera. Figure 7 from Ref. 20 .
Flow cytometry has been shown to distinguish between microorganisms
on the basis of scattering and autofluorescence alone, e.g. discrimination of
yeast and bacteria using light scattering on the basis of size and shape. Changes
in forward-scatter have also allowed for discrimination between excysted and
unexcysted Cryptosporidium oocysts. With fluorescent stains, flow cytometry