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was confirmed using Salmonella spiked samples as well as detection in a
complex matrix, such as milk. 19 This device tested a 1 mL sample by pump-
ing at a flow rate of 50 µL min −1 for 20 min ( Fig. 5.5 ).
Figure 5.4 (a) Computer-assisted design of an integrated microscope, shown in cross-
section. Blue and green arrows mark illumination and emission pathways, respectively.
(b) Image of an assembled integrated microscope. Insets, filter cube holding dichroic
mirror and excitation and emission filters (bottom left), PCB holding the CMOS camera
chip (top right) and PCB holding the LED illumination source (bottom right). The wire
bundles for LED and CMOS boards are visible. Scale bars, 5 mm (a,b). (c) Schematic of
electronics for real-time image acquisition and control. The LED and CMOS sensor each
have their own PCB. These boards are connected to a custom, external PCB via nine
fine wires (two to the LED and seven to the camera) encased in a single polyvinyl chlo-
ride sheath. The external PCB interfaces with a computer via a USB (universal serial bus)
adaptor board. PD, flash programming device; OSC, quartz crystal oscillator; I 2 C, two-
wire interintegrated circuit serial communication interface; and FPGA, field-program-
mable gate array. Source: Figure 1 from Ref. 16 . (For color version of this figure, the reader
is referred to the online version of this topic.)
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