Extracellular Purines in Endothelial Cell Barrier Regulation - Extracellular ATP and Adenosine as Regulators of Endothelial Cell Function - page 45

Fig. 3.2 P1 receptor agonist,
NECA, attenuates the
LPS-induced EC barrier
permeability increase.
HPAEC were challenged with
LPS (100 ng/ml) for 1 h and
then exposed to NECA
(100 nM). Transendothelial
electrical resistance (TER) of
the cell monolayers was
registered and presented after
normalization
Fig. 3.3 Immunostaining for the VE-cadherin (adherent junctional protein) demonstrates that
appreciably more VE-cadherin is recruited to the cell junctions ( arrows ) after treating HPAEC
with purines
MLC 20 and transient dephosphorylation of MLC phosphatase. Immunofluorescence
staining of Purines-stimulated HPAEC revealed dramatic increase in VE-cadherin
expression at the cellular periphery (Fig. 3.3) [54].