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In-Depth Information
A
B
P-UAEC
600
400
1
2
3
4
5
6
100% confluent
200
100
P-UAEC
NP-UAEC
AT P
80
0
0
5
10
15
20
25
30
60
Time/min
*
40
*
NP-UAEC
*
600
20
*
0
400
12345
200
1
2
3
4
Peaks
0
ATP
0
5
10
15
20
25
30
Time/min
Fig. 11.5
Effects of pregnancy on sustained phase Ca
2+
bursts in UAEC. (
A
) Responses to
100
M ATP in individual NP- and P-UAEC at 100% confluency over 30 min. Note the presence
of secondary bursts in each case and the more sustained nature of the secondary bursts in P-UAEC
following the initial 5 min of the response. (
B
) Pregnancy specific changes in likelihood of [Ca
2+
]i
bursts observed in NP- and P-UAEC in response to 100
μ
M ATP. The incidences of [Ca
2+
]i bursts
(numbered as in
A
) were determined for 60 or more cells per field in data from
n
μ
5-7 separate
cell preparations and the percentage of cells responding with each successive burst are shown.
Data are mean
=
SE, with significant difference between NP- and P-UAEC response shown by
∗
P
< 0.05. Figure is modified from that published in [37]
±
11.9 Evidence for Expression of CX43 in UAEC and its Role
in Mediating Cell Synchronization Through Formation
of CX43 Gap Junctions
In view of the findings of cell synchronization, Yi and coworkers sought to identify
the means by which this may occur. In most instances of such behavior in endothe-
lial cells in particular, the mediator is often a Gap junction comprised of a complex
of members of the Connexin family. There are several members that may form
hemichannels on the plasma membrane (to act as pores to release small molecules),
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