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Fig. 10.9 Transduction of the effect of thrombin and adenosine on intercellular communication in
BCEC
presence of the combination of these three protein kinase inhibitors provides evi-
dence that the major pathways for the effect of thrombin on the active area are via
MLCK, Rho kinase and PKC [26].
Our experiments showed that adenosine, which elevates cAMP levels through
activation of G
s -coupled A2B, inhibits the effect of thrombin on IC. Since
PKA inhibits RhoA activation, elevated cAMP causes MLC dephosphory-
lation. Pretreatment of BCEC with adenosine reduced the thrombin-induced
MLC phosphorylation [80]. Therefore, these experiments suggest that adeno-
sine decreases the inhibitory effect of thrombin on ATP release and PIC
by inhibition of RhoA through elevated cAMP, and thereby reduces MLC
phosphorylation [27].
α
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