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Fig. 10.6
Effect of histamine on the Ca
2+
wave propagation. (
aandb
) Normalized Fluorescence
(NF) values of the Ca
2+
transient measured with Fluo-4AM in a typical experiment in the
mechanically stimulated (MS) cell and in neighboring (NB) cell layers NB1-NB5 surrounding the
mechanically stimulated cell. (
a
) in control. (
b
) in the presence of histamine (100
M for 10 min).
(
c
): Active area (AA) of the Ca
2+
wave propagation in control conditions and in the presence of
histamine, and in the presence of histamine after preincubation with
43
Gap26 or
43
Gap27 (300
μ
M
for 30 min). In the presence of
43
Gap26, histamine does not cause a significant further decrease of
the active area. (
N
μ
50)
∗
p
< 0.001 for presence versus absence of histamine. (
d
)Activeareaof
the Ca
2+
wave propagation in control conditions and in the presence of histamine, and in the pres-
ence of histamine after preincubation with apyrase VI + apyrase VII (5 U/ml each). In the presence
of apyrase, histamine does not cause a further decrease of the active area. (
N
=
35).
∗
p
< 0.001
for presence versus absence of histamine. (
e
)ActiveareaoftheCa
2+
wave propagation in control
conditions and in the presence of histamine, and in the presence of histamine after preincubation
with ARL-67156 (100
μ
M for 30 min). The very marked increase in active area by ARL is abol-
ished in the presence of histamine (
N
=
30).
∗
p
< 0.001 for presence versus absence of histamine.
ˆ
p
< 0.001 for ARL vs. control
=
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