Biology Reference
In-Depth Information
Fig.
subunits of ATP synthase on HUVEC surface.
( a ) Nonpermeabilized HUVEC were immunostained with a murine monoclonal antibody directed
against the
9.2 Confocal
images of
α
and
β
subunit of ATP synthase ( green ). ( b ) The same field of HUVEC were immunos-
tained with a rabbit polyclonal antibody directed against the
α
subunit of ATP synthase ( red ). ( c )A
digital overlay of panels a and b demonstrates colocalization ( yellow )of
β
subunits in punc-
tate pattern on surface of HUVEC. ( d ) HUVEC were permeabilized with 100% ethanol prior to
immunostaining with antibodies against the
α
and
β
subunits of ATP synthase. The staining pattern
is characteristic of mitochondrial ATP synthase staining. (Reprinted by permission of Proceedings
of the National Academy of Sciences, Copyright 2001 National Academy of Sciences, USA [25])
α
and
β
Search WWH ::




Custom Search